| 质粒名称: | 3X ERE TATA luc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 11354 |
| 存储实验室: | Donald McDonnell |
|
载体骨架基本信息 |
|
| 载体名称: | pGL2-TATA-Inr |
| 载体出品公司: | Stratagene |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5600 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 细菌表达；Luciferase |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ERE |
| 物种来源: | H. sapiens (human) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BglII(未破坏) |
| 3' 克隆位点: | BglII (未破坏) |
| 5' 测序引物 | lucNrev |
|
文献引用方法 |
|
| 材料和方法部分: | 3X ERE TATA luc was a gift from Donald McDonnell (爱迪基因 plasmid # 11354) |
| 参考文献部分: | The estrogen receptor beta-isoform (ERbeta) of the human estrogen receptor modulates ERalpha transcriptional activity and is a key regulator of the cellular response to estrogens and antiestrogens. Hall JM, McDonnell DP. Endocrinology. 1999 Dec . 140(12):5566-78. 10.1210/en.140.12.5566 PubMed 10579320 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 3X ERE TATA luc 5757 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Luciferase reporter containing three copies of vitellogenin Estrogen
Response Element .
ACCESSION .
VERSION .
KEYWORDS 3X ERE TATA luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5757)
AUTHORS Hall JM, McDonnell DP
TITLE The estrogen receptor beta-isoform (ERbeta) of the human estrogen
receptor modulates ERalpha transcriptional activity and is a key
regulator of the cellular response to estrogens and antiestrogens.
JOURNAL Endocrinology. 1999 Dec . 140(12):5566-78.
PUBMED 10579320
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|3X ERE TATA luc|
FEATURES Location/Qualifiers
source 1..5757
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 387..452
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 582..602
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 1027..1161
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1303..1320)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1474..2062)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1554..1573)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2233..3093)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2856..2875
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3094..3198)
/gene="bla"
/label=AmpR promoter
rep_origin 3225..3680
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3312..3331)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3522..3543
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 3870..3991
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS 4251..146
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
primer_bind complement(4317..4335)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
ORIGIN
1 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
61 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
121 agaagggcgg aaagtccaaa ttgtaaaatg taactgtatt cagcgatgac gaaattctta
181 gctattgtaa tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
241 gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag
301 aaattcgccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
361 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
421 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
481 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
541 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
601 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
661 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
721 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
781 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
841 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
901 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
961 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
1021 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
1081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
1141 gtatcttatc atgtctggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc
1201 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
1261 atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct
1321 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
1381 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
1441 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
1501 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
1561 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
1621 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
1681 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
1741 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
1801 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
1861 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
1921 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
1981 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
2041 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
2101 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
2161 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
2221 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
2281 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
2341 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
2401 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
2461 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
2521 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
2581 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
2641 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
2701 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
2761 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
2821 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
2881 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
2941 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
3001 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
3061 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
3121 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
3181 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
3241 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
3301 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
3361 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
3421 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
3481 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
3541 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
3601 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
3661 aatattaacg cttacaattt gccattcgcc attcaggctg cgcaactgtt gggaagggcg
3721 atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag taagtaatat
3781 taaggtacgt ggaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
3841 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
3901 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
3961 gtggtttgtc caaactcatc aatgtatctt atggtactgt aactgagcta acataacccg
4021 ggaggtaccg agctcttacg cgtgctagct cgagatctag gtcacagtga cctgcggatc
4081 cgcaggtcac tgtgacctag atccgcaggt cactgtgacc tagatctgat atcatcgatg
4141 aattcgggct ataaaagggg gtggggggag ctcggccctc attctggaga cggatcctct
4201 agagtcgacc tgcaggcatg caagcttggc attccggtac tgttggtaaa atggaagacg
4261 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
4321 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
4381 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
4441 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
4501 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
4561 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
4621 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
4681 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
4741 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
4801 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
4861 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
4921 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
4981 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
5041 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
5101 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
5161 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
5221 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
5281 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
5341 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
5401 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
5461 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
5521 cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
5581 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
5641 tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg
5701 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcg
//
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